Effect of Various
Mulches on Soil Chemical Properties and Rhizosphere
Bacteria of Wine Grape (Vitis vinifera)
Peng Jiang1,
Quan Sun1, Junxiang Zhang2,3
and Rui Wang1,2,3*
1College of Agronomy, Ningxia University, Yinchuan 750021, P.R. China
2Ningxia Grape and Wine Research Institute, Yinchuan, Ningxia 750021, P.R.
China
3China Wine Industry Technology Institute, Yinchuan, Ningxia 750021, P.R.
China
*For Correspondence:
amwangrui@126.com
Received 10 May 2021; Accepted 17 January 2022; Published
28 February 2022
Abstract
Mulching has been used widely to
improve the production capacity of the soil, yield and the quality of wine
grapes (Vitis vinifera L. cv. Cabernet Sauvignon). However, the
ecological mechanisms underlying the mulching materials were not well
understood. This study aimed to evaluate the effects of different mulching
materials on soil chemical properties, bacterial community and wine grape
quality. Experiments were performed at a grape-yard on the six-year-old wine
grape ‘Vitis vinifera L. cv. Cabernet sauvignon. Five
kinds of mulching materials include living
turfgrass (Grass), living Herba portulacae
(Por), inorganic plastic black film (Film), organic chips of wood (Wood), and
grape branches (Branch) were applied, while clean tillage (CK) was treated
as a control. Soil chemical properties and grape quality indicators were
measured. Soil bacterial community diversity was detected using the Illumina Miseq sequencing for the 16S rRNA gene V3-V4 region. Mulching
with plastic film, wood chips and Herba portulacae (Por) increased the content of soil organic
matter, available N, P and K, total N and P. Film, grass, branch and Por
mulching materials improved the content of tannin,
anthocyanin, total phenol and titratable acid in grape (p<0.05). Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria and Gemmatimonadetes were
abundant bacteria. Mulching with grape branch and portulacae
increased the relative abundance of Gemmatimonadetes and Chloroflexi phylum, Gemmatimonadaceae family, and decreased Micrococcaceae family and Pseudarthrobacter genus. Mulching with living, organic, or
inorganic changed chemical properties and grape quality indicators. These changes
might be correlated with the altered soil bacterial community diversity and
richness. © 2022
Friends Science Publishers
Keywords: Mulching; Soil chemical
properties; Wine grape quality; 16S rRNA gene sequencing; Rhizosphere bacteria;
Vitis vinifera L. cv. Cabernet sauvignon
Introduction
Mulching is a major practice to reduce soil evaporation,
control salinity and increase crop yield (Aragüés et al. 2014). Many studies have
confirmed the benefits of soil mulching in improving plant growth and yield as
well as in modulating the soil physicochemical properties like soil pH,
moisture, salinity and sodicity, total porosity,
available nitrogen (N) and organic matter (Iiles
1999; Aragüés et al. 2014; Ni et al. 2016; Wang et al. 2017; Qu et al.
2019). Mulching is also effective in the modulation of soil bacterial conmunity (Qian et al. 2015; Farmer et al. 2017; Munoz et al.
2017).
Vitis
vinifera is a worldwide cultivated fruit due to the rising
position of the wine business in the national economy. The texture and aroma of
wine are determined by the varieties, ecological environment, and agricultural
practices (Yuyuen et al. 2015; Urcan et al.
2016; Kok and Bal 2017; Mencarelli and Bellincontro 2018). The contents
of sugar, polyphenols, soluble solids, tannins, phenol compounds and
sugar-acidity ratio determine wine's quality and economic value (Yuyuen et al.
2015; Mencarelli and Bellincontro 2018). These indicators are variable
and robustly influenced by factors like temperature, fertilization, soil
management practices, and microbes (Aragüés et al. 2014; Leeuw et al. 2014; Urcan et al.
2016; Kok and Bal 2017; Huang et al.
2018). Among these factors, soil microbes play a critical role in
regulating processes such as the decomposition of organic matter, nutrient
cycling, as well as disease suppression. Especially, rhizosphere bacteria could
regulate the crop yield and quality, promote plant growth and development via
modulating the root metabolism, absorption, conversion and tolerance to abiotic
stresses (Yang et al. 2009; DeBruyn et al.
2011; Dubey et al. 2019; Ullah et al. 2019). Mulching has been
reported to Table 1: The baseline chemical parameters of soil in our
test plots at the beginning of experiments
Parameters |
0-20 cm |
20-40 cm |
40-60 cm |
pH |
8.32 ± 0.00c |
8.47 ± 0.01a |
8.40 ± 0.00b |
Organic matter (g/kg) |
6.26 ± 0.22a |
5.78 ± 0.34b |
4.82 ± 0.16c |
Available N (mg/kg) |
24.03 ± 0.18a |
21.27 ± 0.56b |
13.93 ± 0.35c |
Available P (mg/kg) |
13.26 ± 0.72a |
8.07 ± 0.39b |
4.09 ± 0.68c |
Available K (mg/kg) |
223.33 ±7.42a |
183.84 ± 2.85b |
117.62 ± 4.57c |
Total N (g/kg) |
0.48 ± 0.02a |
0.44 ± 0.01b |
0.28 ± 0.01c |
Total P (g/kg) |
0.28 ± 0.01a |
0.25 ± 0.01b |
0.17 ± 0.01c |
The significant differences between
the groups are marked by different lowercase letters
play an important role in
controlling the structure of soil bacterial communities (Farmer et al. 2017; Munoz et al. 2017). However, the
relationships between the mulching materials and bacterial communities in the
rhizosphere of grapes were still unclear. Furthermore, the micro-ecological
mechanism under the mulches and yield and quality of the grape needs to be
demonstrated.
There are
many kinds of mulch, divided into organic and inorganic mulch. The organic
mulches consist of animal and plant residues. The most commonly used organic
mulches include straws, husks, grasses, cover crops (live mulches), saw dust,
compost and manures (Iqbal et al. 2020). While the most frequently used inorganic
mulch throughout the world is polyethylene plastic mulch, which may bring
potential environmental pollution (Zhang et al. 2021). Mulches can
potentially reduce weed infestation and evaporation losses and enhance soil's
percolation and retention rate. It was reported that straw mulch could decrease
the rate of evaporation by 35% (Iqbal et al. 2020). Non-living mulch
materials had the greatest capability in moisture conservation in soil compared
to un-mulched soil.
We performed this study to
investigate the soil mulching-induced changed in soil bacterial community
diversity, soil chemical properties and the fruit quality of grape (V. vinifera L. cv. Cabernet sauvignon).
We hypothesized that different organic mulches have different effects on soil.
Illumina Miseq sequencing was performed to determine
the structure and richness of soil bacterial communities. Comparative analyses
were performed to analyze the different effects of mulching materials on soil
bacterial diversity and chemical properties. Our findings would provide a theoretical
basis for the high-quality cultivation of wine grape in dry areas.
Materials
and Methods
Experimental
Field Condition
The experimental site was
located in the wine grape planting base of Lilan
Winery, at the eastern foothills of Helan Mountain, Minning town, Yongning county,
Yinchuan city, Ningxia province, China (latitude 38°16′38′′N, longitude 105°58′20′′ E, above sea level 1129 m), which
is characterized by a temperate arid climate with low rainfall (~200 mm
annually), high evaporation (~1580 mm annually), high total solar radiation
amount (~6100 MJ/km2 annually) and short frost-free period (~176
days). The soil chemical properties are shown in Table 1.
Experimental
Materials and Design
Our experiment was conducted at a grape-yard with
six-year-old V. vinifera L. cv.
Cabernet sauvignon wine was planted over two growing seasons from April 2017 to
October 2018. Grapevines were planted in north-south direction (n = 20 in each
line) with 0.6 m × 3.5 m planting space, with a final density of 4760 plants/hm2.
Thirty experimental plots with 60 grapevines in each plot were randomly divided
into five groups (five kinds of mulches, n=6): (1) turf grass (Grass group),
(2) Herba portulacae (Por
group, sowed with 30 kg/hm2 seeds), (3) plastic black film (Film
group, 0.008 mm thickness), (4) wood chips (Wood group, 4-6 cm in length), (5)
chips of grape branches (Branch group; 1–2 cm in length). The routine clean
tillage (10 cm deep) without mulching was control (CK group). Corresponding
materials covered the soil surface (width of 100 cm) under grapevine for all
treatments. Grapevines were regularly irrigated with dropper facilities and
conventionally fertilized. At the end of October in each year, mulching films
were reclaimed and other mulching materials were buried into soil. The
experimental plots were divided into 5 groups with 6 repetitions.
Soil Chemical Parameters
Measurement
Root rhizospheric soil samples
(5–60 cm in depth) were collected from five randomly selected plants in each
plot. Samples were air-dried, ground, filtered and dissolved into distilled
water (1: 3(v/v) =soil/water). Soil organic matter (organic carbon) was
determined using K2Cr2O7 digestion methods. Available N, P, and K content were
determined using alkaline hydrolysis diffusion methods, 0.5 mol/L NaHCO3-Mo-Sb colorimetry and 0.5 mol/L NaHCO3-flame photometric methods, respectively. Total N
and P content were determined using H2SO4-H2O2
digestion-Nessler's reagent methods and vanadium molybdate yellow colorimetric
method, respectively. All detections were performed following the methods as
previously described by Bao SD (Bao 2000).
Five replications randomly selected from each plot within one group were tested
for each experiment.
Grape Quality Properties
Measurement
Twenty grapes were harvested from each plot in September
2018 and were ground into juice. The solid soluble content was detected
immediately using a MISCO Palm Abbe™ handheld digital refractometer (MISCO
PA201, Misco, Solon, OH, USA). According to the
methods reported by titratable acid, tannin and total phenols content were
detected using NaOH titration methods, Folin-Denis assay and Folin-Ciocalteu
methods (Li
et al. 2000). Anthocyanin content was detected using pH-differential
spectrophotometry (Li et al. 2000). All experiments were performed in 5
replications.
Soil bacterial DNA extraction and
Illumina sequencing
Three Rhizospheric soil samples
(5–60 cm in depth) were collected from each plot (250 mg of each plot х 3
repeats). According to the manufacturer’s instructions, bacterial DNA samples
were extracted from soil samples using a MOBIO PowerSoil
DNA Isolation Kit (MO BIO Laboratories, USA). DNA quality was determined using
a NanoDrop ND-2100 spectrophotometer (NanoDrop Technologies, USA). PCR amplification was
performed using the universal primer pairs (515F/806R with barcode) for the 16S
rRNA gene V3 and V4 regions and TransGen AP221-02 TransStart Fastpfu DNA polymerase
(TransGen Biotech, China). An equal amount of the DNA
samples within one plot were pooled and used to construct a DNA library using a
DNA PCR-Free Sample Preparation Kit (Illumina, USA) according to the
manufacturer’s instructions. Illumina MiSeq platform
with a pair-end (PE) 2х150 bp model was employed for the 16S rRNA gene
sequencing.
Data processing and analysis
Sequencing data (Fastq files) were processed using Trimmomatic
(Bolger et
al. 2014), Pear (P < 0.0001)
(Zhang et
al. 2013), FLASH (http://ccb.jhu.edu/software/FLASH/)
and usearch program (Alloui
et al. 2015) for cleaning the raw data via removing the reads < 50 bp
and barcode reads, data splicing and quality filtering, removing Chimera reads,
respectively. Tags in short length (< 200 bp) were removed using mothur (Yang et al. 2014). Operational
taxonomic units (OTUs) with 97% identity were identified and clustered using Uparse software (Edgar 2013) and single OTUs were removed.
Rarefaction curves, Shannon-Wiener curves, and the species accumulation curves
of the samples were presented using mothur (Yang et al.
2014). The alpha (Chao1, observed OTUs, PD whole tree and
Shannon) of and beta diversity index (Unweighted UniFrac
distance) of each sample was calculated and compared between groups. For the
annotation of OTUs, Ribosomal Database Project (RDP) classifier program (Cole et al.
2008) and the SILVA ribosomal RNA (rRNA) database (Quast et al.
2012) were used. Principal Component Analysis (PCA) was performed for
sample clustering. The relative abundances of OTUs at each taxonomic level were
calculated and different taxonomies among groups were identified using Kruskal-Walli’s test with the threshold of P < 0.05. The cluster analysis tree was built using the genetic
distance UPGMA (Unweighted pair group method with arithmetic mean) algorithm ( Dongen and Winnepenninckx
1996).
Statistical analysis
All data of chemical properties and grape quality
parameters were expressed as the mean ± standard deviation (SD). Analysis of
variance was performed using SPSS 21.0 and multiple comparisons were performed
using the LSD method (α = 0.05) P
< 0.05 was considered as significant difference.
Results
Mulching methods effect
on soil chemical properties and wine grape quality
This study showed that mulching with plastic
film, grass, wood chips, and herba portulacae (Por) increased the contents of soil organic
matter and available N significantly (P < 0.05). But mulching with grapevine
branch decreased soil organic matter, available N and P compared with CK and
other mulching materials (P < 0.05,
Table 2). Grass mulching decreased soil available P and K (significantly, P < 0.05) and total N versus CK (insignificantly,
P > 0.05, Table 2). Mulching increased tannin, anthocyanin,
total phenols and titratable acid but decreased soluble solid contents in wine
grape (P < 0.05, Table 3). These
results suggested that plastic film and organic mulching materials like wood
and portulacae were efficacious in improving soil
fertility and might be recommendable agricultural practices for improving the
soil cultivability. Evidently, mulching materials like wood significantly
increased the Total N (increased by 63%) and Total P (increased by 21%). Por
significantly increased the OM and Available N by 42 and 85%, respectively. The
use of grass and grapevine branches for mulching showed uncertain efficacies in
decreasing the soil cultivability.
General analysis of the Illumina 16s rRNA gene sequencing data
To investigate the effect of mulching on the modulation
of edaphology, Illumina 16S rRNA sequencing was performed to detect the
bacterial community diversity in rhizosphere soil. Illumina Miseq sequencing generated 1,550,850 raw tags, including
536,970 clean tags (Table S1). Most (98.76%) of these tags were in the length
of 400~440 bp (Fig. 1a). In total 78,182 OTUs were
identified, with an average number of 2,606 tags per sample (Table S2). The
rarefaction curves of samples sequenced showed that higher numbers of OTUs might
Table 2: Mulching methods effect on soil chemical
properties in maturity stage
Group |
Organic
matter (g/kg) |
Available
N (mg/kg) |
Available
P (mg/kg) |
Available
K (mg/kg) |
Total N
(g/kg) |
Total P
(g/kg) |
CK |
7.27 ± 0.22c |
25.59 ±
0.13d |
6.63 ± 0.12c |
217.20 ±
0.58cd |
0.46 ± 0.00e |
0.28 ± 0.01b |
Film |
9.73 ± 0.15b |
40.18 ±
2.20b |
18.14 ±
0.55a |
311.63 ±
1.60a |
0.59 ± 0.00d |
0.31 ± 0.01ab |
Grass |
7.58 ± 0.08c |
32.96 ±
0.56c |
5.12 ± 0.25d |
208.33 ±
0.45d |
0.39 ± 0.01f |
0.35 ± 0.03a |
Wood |
9.62 ± 0.06b |
34.23 ±
1.49c |
5.10 ± 0.22d |
313.89 ±
11.07a |
0.75 ± 0.01a |
0.34 ± 0.01a |
Branch |
5.59 ± 0.01d |
24.21 ±
0.39d |
3.38 ± 0.21e |
224.00 ±
3.03c |
0.66 ± 0.00c |
0.29 ± 0.00b |
Por |
10.32 ±
0.06a |
47.46 ±
0.65a |
11.74 ±
0.23b |
289.60 ±
0.24b |
0.69 ± 0.01b |
0.31 ± 0.01ab |
The significant differences between the groups are marked by different
lowercase letters. Por, herba portulacae
Table 3: Mulching methods effect on the quality of wine
grape berry
Group |
Tannin (mg/kg) |
Anthocyanin
(mg/kg) |
Total
phenols (mg/kg) |
Soluble
solid (%) |
Titratable
acid (%) |
CK |
13.81 ±
0.22c |
5.71 ± 0.03e |
16.02 ±
0.21c |
25.56 ±
0.14a |
0.62 ± 0.01b |
Film |
17.30 ±
0.41a |
7.29 ± 0.01c |
20.06 ±
0.55a |
23.24 ±
0.33c |
0.70 ± 0.01a |
Grass |
16.62 ±
0.37a |
7.58 ± 0.05c |
20.29 ±
0.25a |
25.22 ±
0.45a |
0.71 ± 0.01a |
Wood |
12.52 ±
0.14d |
8.49 ± 0.19a |
18.98 ±
0.22b |
25.24 ±
11.07a |
0.65 ± 0.01b |
Branch |
14.73 ±
0.19b |
6.63 ± 0.08d |
16.70 ±
0.21c |
24.48 ±
3.03ab |
0.73 ± 0.01a |
Por |
17.25 ±
0.26a |
8.06 ± 0.23b |
19.93 ±
0.23a |
23.80 ±
0.24bc |
0.72 ± 0.01a |
The significant differences (P < 0.05) between the groups are
marked by different lowercase letters. Por, herba portulacae
Fig.
1: The OTUs distribution and curves of samples sequenced. (a) the length distribution of the OTUs,
(b) the rarefaction curves of the
samples showing the depth of sequencing and the possibility of OTUs numbers, (c) the Shannon curves showing the depth
of sequencing and the possibility of bacterial diversity, (d) Specaccum species cumulative curve
showing the increase rete of new species with sequencing size
be produced with deeper sequencing (Fig. 1b).
Shannon-Wiener curves showed that deeper sequencing would not increase the
bacterial diversity and the present Illumina sequencing data was sufficient for
diversity analysis (Fig. 1c). Species accumulation curves revealed that the
sample size was sufficient to reflect the richness of the community (Fig. 1d).
Further analysis showed there were no differences in the alpha diversity
indicators (e.g., Chao1, observed
OTUs, PD whole tree and Shannon) among groups (Fig. 2a–d) and the unweighted_unifrac_distance in each group (Fig. 3a). PCA
showed that samples in the Wood, Grass and Por groups were not discriminant,
while the clusters of other samples in the CK, Film and Branch groups were
relatively compact (Fig. 3b). Based on the annotation and abundance calculation
of OTUs, we observed some OTUs or bacteria were differentially distributed in
the samples (Fig. 4). Accordingly, we identified the differential rhizosphere
bacteria in response to the mulching methods.
Identification of the differential bacteria by different mulching
materials
Proteobacteria, Actinobacteria, Chloroflexi, Acidobacteria, Gemmatimonadetes and Bacteroidetes
were abundant in all groups (4.46~24.28%; Fig. 5a and supplementary Table S3). Gemmatimonadaceae
(Gemmatimonadetes),
Micrococcaceae (Actinobacteria),
Anaerolineaceae
(Chloroflexi)
and Cytophagaceae
(Bacteroidetes) were abundant
bacteria at the family level (Fig. 5b and supplementary Table S4). The relative
abundance of Gemmatimonadetes
phylum and Gemmatimonadaceae
family was increased by mulching grape branches (from 8.77
± 0.37 to 10.46 ± 0.18% P < 0.05
and from 4.44 ± 0.21 to 7.04 ± 0.75%, P
<0.05, respectively) and herba portulacae (from 8.77 ± 0.37 to 9.91 ± 0.67%, P > 0.05, from 4.44 ± 0.21 to 5.83 ± 0.65%,
P < 0.05, respectively; Fig. 5c
and d). Grass mulching decreased Gemmatimonadetes phylum (from 8.77 ±
0.37 to 6.88 ± 0.30%, P <0.05) and
Micrococcaceae family (from 4.33 ± 0.47 to 3.47 ± 0.08%,
P <0.05, Fig. 5d). The abundant
genus Pseudarthrobacter
(Actinobacteria, 3.79 ± 0.97%) were
decreased in branch (1.34 ± 0.79%, P <
0.05) and Por group (1.31 ± 1.24%, P <
0.05) and abundant genus Sphingomonas (Proteobacteria,
1.54 ± 0.20%) were decreased in Film (0.91 ± 0.22%, P < 0.05) and Grass group (1.06 ± 0.04%, P < 0.05; Fig. 6a–b and supplementary Table S5).
Discussion
Soil mulching could improve the soil chemical parameters
(including organic matter, available NPK and total NP). Materials of grape
branches and herba portulacae
changed the community structure of soil bacteria. Mulching materials showed
multiple benefits in plant growth via regulating soil temperature, moisture,
total porosity and organic matter, and decreasing soil evaporation. The
efficient effect of mulching materials on ecological restoration and the soil
physicochemical properties showed mulching had important roles in regulating
and modulating the edaphology. It has been reported that the soil bacterial
diversity could be altered by the soil physicochemical properties (Farmer et al.
2017). Our study found that mulching could improve the soil chemical
parameters, such as organic matter, available NPK and total NP. Hence, we
confirmed the effect of mulching on soil physicochemical properties and the
influence of mulching on soil bacteria community diversity.
It has been reported that mulching
with wood chips significantly promoted the growth of plant as well as improved
the available N and organic matter in soil, mulching with organic materials
(like green waste compost and pine bark) for a long-term (2-years) increased
the soil organic matter, total N, mineral N and available P and K, while
mulching with turf grass only increased the soil total N and available K,
suggesting the weak contribution of grass mulching to soil fertility. These
results in the above reports were inconsistent with the results in our studies
that grass and branch materials showed questionable effects on soil fertility.
In this present study, mulching significantly increased the soil chemical
properties. We speculate that these differences may be due to the differences
in depth and soil type (Duryea et al. 1999; Iiles 1999; Ni et al. 2016). Also, other reports
showed that the organic mulches significantly decreased the pH value in soil of
fine sandy loam (Billeaud and Zajicek 1989; Duryea et al. 1999; Wang et al. 2017), whereas some showed opposite opinions (Iiles 1999; Ni et al. 2016). Despite the
aforementioned differences, our present study confirmed that mulching with
plastic film and herba portulacae
had high efficacies in improving the fertility of mixed soil samples.
Mulching showed considerable
efficacy in plant growth and crop quality (Qu et al. 2019). Ni et al. (2016)
reported that mulching with different materials improved plant height, root
activity, electric conductivity and the content of chlorophyll a/b, water,
soluble sugar and proline in leaves. However, revealed that mulching did not
significantly change the growth and height of Sophora japonica. Here in our present study, mulching improved
grape quality indicators (e.g.,
tannin, anthocyanin, total phenols and titratable acid) but decreased soluble
solid contents in wine grape.
Mulching-induced changes in soil physicochemical
properties may significantly influence bacterial diversity or richness (Aragüés et al.
2014; Farmer et al. 2017). The
soil bacterial community composition here was accorded
Fig. 2: The alpha diversity indicators. Difference in alpha diversity indicators
including Chao1 (a), observed OTUs (b), PD whole tree (c) and Shannon (d) is
analyzed by Kruskal-Walli’s test. Grass, soil surface
was mulched with natural grass (< 5 cm); Por, herba
portulacae; Film, black plastic film; Wood, wood
chips; Branch, chips of dry grape branches; CK, with nothing but clean tillage
Fig. 3: Beta diversity analysis and Principal Component Analysis (PCA). (a) boxplot of the Unweighted UniFrac distance in groups, (b) the PCA scattered plots of samples sequenced
Fig. 4: The heatmaps of the relative
abundance of the top 20 phylum (a)
and top 20 OTUs (b). Red indicates
high relative abundance, and blue notes low relative abundance of the related
bacterial phylum (a) or OTUs (b) in each sample sequenced
Fig. 5: Relative abundance of OTUs of the dominant
bacteria at phylum and family level. (a)
and (b) the stacks of OTUs’ relative
abundance of the dominant phyla and family, respectively. (c) and (d) the
statistical analysis for OTUs’ relative abundance of the top 10 phyla and
family, respectively. * P < 0.05 vs. CK group. All differences were
called by Kruskal-Walli’s test
Fig. 6: The stacks (a) and statistical
analysis (b) for OTUs’ relative
abundance of the dominant genera. * P
< 0.05 vs. CK group. All
differences were called by Kruskal-Walli’s test
with the reported fact that Proteobacteria, Actinobacteria,
Chloroflexi,
Acidobacteria,
Gemmatimonadetes and Bacteroidetes
were dominant soil bacteria (Janssen et al. 2002; Spain et al. 2009; Davis et al.
2011; Miyashita 2015). Detected that the abundance of Chloroflexi and
the soil enzyme activity which linked to the organic matter decomposition (e.g., β-glucosidase, β-D-cellobiosidase, Phosphatase and N-acetyl-β-D-glucosaminidase)
were increased in soil at the same time (Delgado-Baquerizo et al. 2018). This present study
found that the relative abundance of Chloroflexi phylum was decreased by mulching with the
chips of grape branches and herba portulacae.
We speculated that there might be a
positive association between the Chloroflexi bacteria and soil organic matter decomposition.
Here in our present study, we demonstrated that the relative abundance of Chloroflexi phylum was decreased by mulching with the chips of grape branches and herba portulacae. The decreased Chloroflexi
phylum was not in line with the soil organic matter content in the two groups,
suggesting there might not have a direct link between Chloroflexi phylum abundance and organic
matter decomposition.
Many of the soil bacteria, like Actinobacteria and Gemmatimonadaceae, are related to
the tolerance or defense against stresses (Marschner et al. 2003; DeBruyn et al. 2011; Yandigeri et al. 2012; Ullah et al. 2019). For instance, the dynamic changes of Gemmatimonadetes
with temperature and time in terrestrial systems implicated that the crucial
role of these environmental factors in soil ecology systems (DeBruyn et al.
2011). Accordingly, much effort has been performed to evaluate the
influence of agricultural practices on improving soil cultivability or plant
tolerance by regulating the communities of bacteria (Jamieson et al. 2002; Marschner et al. 2003; Yang et al. 2009; Fawaz 2013; Zolla
et al. 2013; Ullah et al. 2019).
Phylum Gemmatimonadetes
is one of the top 10 soil bacteria (DeBruyn et al. 2011). Some studies have
shown that the abundant Gemmatimonadetes
phylum could be influenced by soil organic matter content, drought degree, and
N content (DeBruyn et al. 2011). Ullah et al. (2019) reported that Gemmatimonadaceae
was dominant in the drought-treated rhizosphere. DeBruyn et al. (2011)
reported that the abundance of Gemmatimonadetes in a desert or arid soil were higher than
those from the forest or pasture. The increased abundance of Gemmatimonadaceae
might be related to the plant tolerance to abiotic stresses like drought and
heat (Ullah
et al. 2019). In our present study, we found that the relative
abundances of Gemmatimonadetes
phylum and Gemmatimonadaceae
family were increased by branch and herba portulacae mulching. It has been reported that the soil
moisture and the soil temperature could be improved by soil mulching (Ni et al.
2016; Gu et al. 2017; Tan et al. 2017; Wang et al. 2017; Qu et al.
2019). Accordingly, we assumed that the increased Gemmatimonadetes and Gemmatimonadaceae
here might not be induced by the drought, but by the increased temperature.
At last, we found the two genera Pseudarthrobacter
and Sphingomonas
were influenced by mulching. Pseudarthrobacter and Sphingomonas belongs to the
subdivision of Proteobacteria and Actinobacteria, respectively and the
latter was dominant soil bacteria with relative constant abundances in diverse
soil types (Janssen et al. 2002; Spain et al.
2009; Davis et al. 2011; Miyashita
2015). This was in consistent with the fact that the Mircrococcaceae family was decreased in branch and Por
group versus control. These results indicated the important roles of these
bacteria in soil ecology and in the growth, development and defense of plants.
Conclusion
Mulching could improve the soil chemical parameters
(including organic matter, available NPK and total NP). However, mulching with
inorganic (black plastic film), organic materials (wood chips and chips of
grape branches) and living (turf grass and herba portulacae) all improved the contents of tannin,
anthocyanin, total phenol and titratable acid in wine grape, but decreased
soluble solid content. Soil mulching materials or the chips of grape branches
and herba portulacae
changed soil bacteria's community structure, including increased Gemmatimonadetes phylum, Chloroflexi
phylum and Gemmatimonadaceae family, which
were reported to be associated with the plant defense or tolerance to abiotic
stresses. The altered abundance of these bacteria indicated the improvement in
the resistance to abiotic stresses in plants by mulching materials.
Acknowledgement
This study was carried out with the assistance of the
National Key Research and Development Project (2019YFD1002500), Ningxia Natural
Science Foundation (2020AAC02011), and technology reform and development
project (106001000000150012).
Author Contributions
PJ and RW: proposed the research
and finalizing the manuscript, QS and JZ: data collection, PJ and RW: DNA
analysis and drafted the manuscript. All authors provided critical feedback and
helped to shape the manuscript.
Conflict of Interest
All authors declared there were no
conflicts of interest involved.
Ethics Approval
Not applicable
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